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Composite

Part:BBa_K2016006:Design

Designed by: Magdalena Dabrowska   Group: iGEM16_Sheffield   (2016-10-07)


Superfolder GFP under control or medium constitutive promoter and RyhB siRNA domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 514
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 38


Design Notes

Cloned between XbaI and SpeI sites.


Source

source

References